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Estimation of total chlorophyll by Aron's method

Purpose:- Estimation of total chlorophyll by Arnon's method. Requirement:- Spectrophotometer Buchner's funnel, filter paper test tube, Fresh leaves. measuring cylinder pestle & mortar 80% Acetone  Formula:- 20.2 (A645) +802 A663) / 1000 x W Principle:- Chlorophyll is extracted in 80% acetone & the absorption at 660 nm& 645 nm are read in s spectrophotometer using the absorption co-efficient, the amount of chlorophyll is calculated Procedure:- 1) Weigh 1 gm of finely cut & well mixed representative sample of a leaf or fruit tissue into a clean mortar 2) Grind the tissue to a fine pulp with the addition of 20mil of 80% acetone   3) Centrifuge (5000 rpm for 5 min) & transfer the supernatant to a 100 ml volumetric flask  4) Repeat this procedure until the residue is colorless wash the mortar and pestle  thoroughly with 80% acetone and collect the clear washing in the volumetric flask 5) Make up the volume to 100 ml with 80% acetone  6) ...

Two staged RC coupled Amplifier

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Purpose: - (a) Study the amplifier combined with two RC components. (b) Set the curve of the frequency response. Therefore determines the bandwidth.   Requirements: - Transistor, function generator, resistors, capacitors, controlled power supply, multimeter, breadboard, connecting cables, etc.   Circuits Diagram:- Opinion: - The amplifier is the building block for many electrical systems. A single-stage amplifier is not enough to build an effective electronic system. When no. The amplifier class is used sequentially called multistage. Amplifier (or) amplifier with cascaded high gain can be obtained from multistage amplifying. Demonstrates how to pair a phase 2 amplifier using the RC-coupling system. This is the most widely used method. In this system, a signal built across the resistor RE (R2) of the first phase is connected below the second phase with a capacitor CE (C2). The coupling capacitor prevents the DC power of the first phase from reaching the base ...

Pre-Emphasis & De-Emphasis Networks

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Aim:-  To draw the frequency response of Pre-emphasis & De-emphasis networks. Introduction:- Educational trainer a useful kit for the demonstration of Pre-emphasis & De-emphasis at different time constants & plot the graph of the frequency response of Pre-emphasis and De-emphasis networks. The kit consists of 200 Hz-20 kHz audio oscillator 70µ sec & 50µ sec De-emphasis networks.  Pre-Emphasis Circuit Diagram:- DE-Emphasis Circuit Diagram:- Theory:- Pre-emphasis refers to boosting the relative amplitudes of the modulating voltage for higher audio frequencies from 2 to approximately 15 kHz . De-emphasis means attenuating those frequencies by the amount by which they are boosted.  However, Pre-emphasis is done at the transmitter and De-emphasis is done in the receiver. The purpose is to improve the signal-to-noise ratio for FM reception. A time constant of 75µ sec is specified in the RC or L/Z network for Pre-emphasis and De-emphasis. Tabular colu...

Addition of two 16-bit numbers

  Aim:- Addition of two 8-bit numbers. Apparatus:- Microprocessor kit. Data:- 7501-98H, LSB's of 1st number 7502-5B, MSB's of 1st number 7503-4CH, LSB's of 2nd number 7504-8EH, MSB's of 2nd number   Memory address   Machine code   Labels     Mnemonics   Operand   Comments   7000   2A,01,75     LHLD   7-501H   1 st   16-bit no. in H-L pair   7003   EB     XCHG     Get 1 st  no. D-E pair   7005   2A,03,75     LHLD   7503H   2 nd  16-bit no. in H-l pair   7007   0E,00     MVI ...